Supplier: Hannes Schihada
Affiliaton: Karolinska Institutet, Stockholm, Sweden
E-mail: Hannes.schihada@ki.se
Alternative Contact Person: Gunnar Schulte, Karolinska Institutet, Stockholm, Sweden (gunnar.schulte@ki.se)
Puplication status: published
Link For Publication: https://doi.org/10.1126/scisignal.abf1653
Link For External Location: Open Access preprint: https://doi.org/10.1101/2021.02.05.429900 Available at the plasmid depository Addgene: https://www.addgene.org/Gunnar_Schulte/
Brief Description: G protein biosensors that reveal G protein activation and heterotrimer dissociation through a drecrease in BRET between NanoLuciferase-tagged Ga and cpVenustagged Gbg
Keywords: GPCR, biosensor, BRET, G protein
Annex: -fluorescence intensity, luminescence intensity, absorbance, FRET, time resolved FRET (TR-FRET), BRET, electrical/impedance, refractive index
-conformational/intramolecular sensor, protein-protein interaction, dynamic mass redistribution (DMR),
-tricistronic plasmid encoding unlabelled Gb, cpVenus-labelled Gg and NanoLuciferase-labelled Ga
-cAMP, Ca2+, DAG, ERK phosphorylation, receptor conformational changes, G protein dissociation, ligand-receptor binding